Regulatory

Part:BBa_K3190001

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-12)


pTDH3: Yeast strong constitutive promoter

The promoter also called GPD promoter regulates the gene for glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH is found in the cytoplasm and the cell wall. It is involved in glycolysis and gluconeogenesis, where it catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate (and the reverse reaction). The TDH3 promoter is used to drive the expression of the TPST1 and TPST2 (Tyrosylprotein sulfotransferase 1 and 2), an enzyme necessary for the relative post-translational modifications of LHCGR receptor. The enzyme catalyzes the sulfation reaction of protein tyrosines.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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